In the present study, Coleus forskohlii plant was subjected to different water stress conditions under controlled temperature in climate control Greenhouses. The roots of the plant were investigated for its biochemical parameters viz. forskolin, chlorophyll, proline and potassium percentage at variable water stress levels. The results revealed that the forskolin and proline content were found to be maximum in the roots of plants with 80% moisture stress. Similarly, the potassium content was also influenced by the water stress treatment. However, the chlorophyll content was found to be maximum in plants with 20% moisture stress. Consequent upon the interpretation of findings it can be concluded that the soil moisture stress has a significant influence on forskolin and proline content. Hence, the crop may be cultivated in a stress condition for maximum production of pharmaceutically important constituent i.e. forskolin.