Hippophae rhamnoides turkestanica (HRT)is capable of withstanding harsh climatic conditions prevalent at high altitude. Various medicinal and nutritional properties of the leaf-extract of this plant have already been established. In vitro studies of the extract ascertained the presence of various phyto constituents. In the present study adaptogenic and immunomodulatory potentials of different fractions of the whole leaf aqueous extract of HRT (SBT-5) are compared. This study was carried out on five fractions of SBT-5. Out of thefive fractions, polyphenolic rich fraction (PRF) showed 66.67% resistance against Cold-Hypoxia-Restraint(CHR) induced multiple stress at a four times lower dose compared to SBT-5. Supplementation of PRF significantly reduced the oxidative stress at tissues levels also. Successive Methanolic fraction amplified the immune response with Ovalbumin and Tetanus Toxoid antigens. As compared to SBT-5, PRF showed better antioxidant and adaptogenic potentials at four-time lower dose. This concludes that HRT fractions show improved performance under multiple stressful conditions as compared to the whole extract.